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p braf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p braf
    JD-02 inhibits HSV-1 replication by suppressing the Raf/MEK/ERK signaling pathway. (A) Western blot analysis was conducted to assess the effects of HSV-1 (MOI=0.1) infection on the protein levels of <t>BRAF,</t> MEK and ERK, with and without treatment using JD-02. (B) Treatment with JD-02 at the specified concentration influences the protein levels of BRAF, MEK and ERK in HaCaT cells over a 12-h period. (C and D) HaCaT cells were subjected to transfection with either N.C. siRNA or ERK siRNA for a period of 48 h. Subsequently, the cells were infected with HSV-1 (MOI=0.1) for an additional 24 h. The DNA copy number of the viral gene UL54, as well as the viral protein expression of gB, ICP0, ICP27, ERK and p-ERK were evaluated. (E) The DNA copy numbers of the viral genes UL30 , UL52 and UL54 in HaCaT cells infected with HSV-1 (MOI=0.1) and subsequently treated with either JD-02 (1 μ M) or U0126 (10 μ M) for 24 h, were quantified using reverse transcription-quantitative PCR. (F) Western blot analysis of viral proteins (gB, ICP0 and ICP27), ERK and p-ERK expression in HaCaT cell infected with HSV-1 (MOI=0.1) and treated with U0126 for indicated concentration. (G) Western blot analysis of UL30 overexpression in HaCaT cells treated with U0126. Data are presented as the mean ± SD (n=3). * P<0.05, ** P<0.01, *** P<0.01 and **** P<0.0001 compared with the HSV-1 group. HSV, Herpes Simplex Virus; MOI, multiplicity of infection; N.C., negative control; siRNA, small interfering RNA; p-, phosphorylated.
    P Braf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+p+braf/Phospho-B-Raf+(Ser445)+Antibody/pmc13042271-96-40-45
    Average 95 stars, based on 149 article reviews
    p braf - by Bioz Stars, 2026-10
    95/100 stars

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    1) Product Images from "Novel Hsp90 inhibitor JD-02 inhibits HSV-1 infection via the Raf/MEK/ERK signaling pathway"

    Article Title: Novel Hsp90 inhibitor JD-02 inhibits HSV-1 infection via the Raf/MEK/ERK signaling pathway

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2026.5810

    JD-02 inhibits HSV-1 replication by suppressing the Raf/MEK/ERK signaling pathway. (A) Western blot analysis was conducted to assess the effects of HSV-1 (MOI=0.1) infection on the protein levels of BRAF, MEK and ERK, with and without treatment using JD-02. (B) Treatment with JD-02 at the specified concentration influences the protein levels of BRAF, MEK and ERK in HaCaT cells over a 12-h period. (C and D) HaCaT cells were subjected to transfection with either N.C. siRNA or ERK siRNA for a period of 48 h. Subsequently, the cells were infected with HSV-1 (MOI=0.1) for an additional 24 h. The DNA copy number of the viral gene UL54, as well as the viral protein expression of gB, ICP0, ICP27, ERK and p-ERK were evaluated. (E) The DNA copy numbers of the viral genes UL30 , UL52 and UL54 in HaCaT cells infected with HSV-1 (MOI=0.1) and subsequently treated with either JD-02 (1 μ M) or U0126 (10 μ M) for 24 h, were quantified using reverse transcription-quantitative PCR. (F) Western blot analysis of viral proteins (gB, ICP0 and ICP27), ERK and p-ERK expression in HaCaT cell infected with HSV-1 (MOI=0.1) and treated with U0126 for indicated concentration. (G) Western blot analysis of UL30 overexpression in HaCaT cells treated with U0126. Data are presented as the mean ± SD (n=3). * P<0.05, ** P<0.01, *** P<0.01 and **** P<0.0001 compared with the HSV-1 group. HSV, Herpes Simplex Virus; MOI, multiplicity of infection; N.C., negative control; siRNA, small interfering RNA; p-, phosphorylated.
    Figure Legend Snippet: JD-02 inhibits HSV-1 replication by suppressing the Raf/MEK/ERK signaling pathway. (A) Western blot analysis was conducted to assess the effects of HSV-1 (MOI=0.1) infection on the protein levels of BRAF, MEK and ERK, with and without treatment using JD-02. (B) Treatment with JD-02 at the specified concentration influences the protein levels of BRAF, MEK and ERK in HaCaT cells over a 12-h period. (C and D) HaCaT cells were subjected to transfection with either N.C. siRNA or ERK siRNA for a period of 48 h. Subsequently, the cells were infected with HSV-1 (MOI=0.1) for an additional 24 h. The DNA copy number of the viral gene UL54, as well as the viral protein expression of gB, ICP0, ICP27, ERK and p-ERK were evaluated. (E) The DNA copy numbers of the viral genes UL30 , UL52 and UL54 in HaCaT cells infected with HSV-1 (MOI=0.1) and subsequently treated with either JD-02 (1 μ M) or U0126 (10 μ M) for 24 h, were quantified using reverse transcription-quantitative PCR. (F) Western blot analysis of viral proteins (gB, ICP0 and ICP27), ERK and p-ERK expression in HaCaT cell infected with HSV-1 (MOI=0.1) and treated with U0126 for indicated concentration. (G) Western blot analysis of UL30 overexpression in HaCaT cells treated with U0126. Data are presented as the mean ± SD (n=3). * P<0.05, ** P<0.01, *** P<0.01 and **** P<0.0001 compared with the HSV-1 group. HSV, Herpes Simplex Virus; MOI, multiplicity of infection; N.C., negative control; siRNA, small interfering RNA; p-, phosphorylated.

    Techniques Used: Western Blot, Infection, Concentration Assay, Transfection, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Over Expression, Virus, Negative Control, Small Interfering RNA

    Related Articles

    Western Blot:

    Article Title: KSR1 knockout mouse model demonstrates MAPK pathway's key role in cisplatin- and noise-induced hearing loss
    Article Snippet: .. The following 203 antibodies were used for immunoblot analysis: anti-BRAF (14814S), anti-p-BRAF (Ser445, 2696S), anti-ERK1/2 (4695), 204 anti-p-ERK1/2 (Thr202/Tyr204, 9101S), anti-MEK1/2 (9122S), and anti-p-MEK1/2 (Ser217/221, 41G9S) were obtained 205 from Cell Signaling Technologies, and anti-α Tubulin (T9026) from Millipore sigma. ..

    Article Title: KSR1 knockout mouse model demonstrates MAPK pathway’s key role in cisplatin- and noise-induced hearing loss
    Article Snippet: .. The following antibodies were used for immunoblot analysis: anti-BRAF (14814S), anti-p-BRAF (Ser445, 2696S), anti-ERK1/2 (4695), anti-p-ERK1/2 (Thr202/Tyr204, 9101S), anti-MEK1/2 (9122S), and anti-p-MEK1/2 (Ser217/221, 41G9S) were obtained from Cell Signaling Technologies, and anti-α Tubulin (T9026) from Millipore sigma. ..

    Article Title: KSR1 Knockout Mouse Model Demonstrates MAPK Pathway’s Key Role in Cisplatin- and Noise-induced Hearing Loss
    Article Snippet: .. The following antibodies were used for immunoblot analysis: anti-BRAF (14814S), anti-p-BRAF (Ser445, 2696S), anti-ERK1/2 (4,695), anti-p-ERK1/2 (Thr202/Tyr204, 9101S), anti-MEK1/2 (9122S), and anti-p-MEK1/2 (Ser217/221, 41G9S) were obtained from Cell Signaling Technology and anti-α Tubulin (T9026) from MilliporeSigma. ..

    Planar Chromatography:

    Article Title: Phthalates promote lenvatinib resistance in hepatocellular carcinoma through the HPX-MAPK pathway.
    Article Snippet: .. The following cell lines, antibodies and chemicals were used in this study: LM3, 97H, 97 L, PLC/PRF/5 (Typical Cultures Preserved Cell Bank, Chinese Academy of Sciences), anti-HPX (Abclonal, A9133, Wuhan, China), anti-GAPDH (Abclonal, A19056, Wuhan, China), antiβ-actin (Starter, S0B0734, Hangzhou, China), anti-p-Braf (CST, 2696, Danfoss, USA), anti-Braf (CST, 14814, Danfoss, USA), anti-p-MEK1/2 (Abclonal, AP1349, Wuhan, China), anti-MEK1/2 (Abclonal, A4868, Wuhan, China), anti-p-ERK1/2 (Abclonal, AP0974, Wuhan, China), anti-ERK1/2 (Abclonal, A4782, Wuhan, China), HRP-coupled goat antirabbit IgG (H+L) (Abclonal, AS014, Wuhan, China), CCK8 assay kit (Beyotime, C0038, Shanghai, China), BeyoClick EdU-488 Cell Proliferation Assay Kit (Beyotime, C0071STM, Shanghai, China), the Whole Protein Extraction Kit (KeyGEN BioTECH, KGB5303–100, Nanjing, China), diethyl phthalate (Merck, 524972, darmstadt, Germany), dimethyl phthalate (Merck, 525081, Darmstadt, Germany), Dioctyl phthalate (Merck, D201154, Darmstadt, Germany), anti-p-MEK1/2 (HUABIO, ET1609–50, Hangzhou, China), anti-p-ERK1/2 (HUABIO, ET1610–13, Hangzhou, China), anti-JNK (HUABIO, ET1601–28, Hangzhou, China), anti-p-JNK (HUABIO, ET1609–42, Hangzhou, China), anti-p38 (HUABIO, ET1702–65, Hangzhou, China), anti-p-p38 (HUABIO, HA722669, Hangzhou, China), MG132 (MCE, HY-13259, New Jersey, USA), bortezomib (MCE, HY-10227, New Jersey, USA), HCQ (MCE, HY-B1370, New Jersey, USA), Bafilomycin A1 (MCE, HY-100558, New Jersey, USA). ..

    CCK-8 Assay:

    Article Title: Phthalates promote lenvatinib resistance in hepatocellular carcinoma through the HPX-MAPK pathway.
    Article Snippet: .. The following cell lines, antibodies and chemicals were used in this study: LM3, 97H, 97 L, PLC/PRF/5 (Typical Cultures Preserved Cell Bank, Chinese Academy of Sciences), anti-HPX (Abclonal, A9133, Wuhan, China), anti-GAPDH (Abclonal, A19056, Wuhan, China), antiβ-actin (Starter, S0B0734, Hangzhou, China), anti-p-Braf (CST, 2696, Danfoss, USA), anti-Braf (CST, 14814, Danfoss, USA), anti-p-MEK1/2 (Abclonal, AP1349, Wuhan, China), anti-MEK1/2 (Abclonal, A4868, Wuhan, China), anti-p-ERK1/2 (Abclonal, AP0974, Wuhan, China), anti-ERK1/2 (Abclonal, A4782, Wuhan, China), HRP-coupled goat antirabbit IgG (H+L) (Abclonal, AS014, Wuhan, China), CCK8 assay kit (Beyotime, C0038, Shanghai, China), BeyoClick EdU-488 Cell Proliferation Assay Kit (Beyotime, C0071STM, Shanghai, China), the Whole Protein Extraction Kit (KeyGEN BioTECH, KGB5303–100, Nanjing, China), diethyl phthalate (Merck, 524972, darmstadt, Germany), dimethyl phthalate (Merck, 525081, Darmstadt, Germany), Dioctyl phthalate (Merck, D201154, Darmstadt, Germany), anti-p-MEK1/2 (HUABIO, ET1609–50, Hangzhou, China), anti-p-ERK1/2 (HUABIO, ET1610–13, Hangzhou, China), anti-JNK (HUABIO, ET1601–28, Hangzhou, China), anti-p-JNK (HUABIO, ET1609–42, Hangzhou, China), anti-p38 (HUABIO, ET1702–65, Hangzhou, China), anti-p-p38 (HUABIO, HA722669, Hangzhou, China), MG132 (MCE, HY-13259, New Jersey, USA), bortezomib (MCE, HY-10227, New Jersey, USA), HCQ (MCE, HY-B1370, New Jersey, USA), Bafilomycin A1 (MCE, HY-100558, New Jersey, USA). ..

    Proliferation Assay:

    Article Title: Phthalates promote lenvatinib resistance in hepatocellular carcinoma through the HPX-MAPK pathway.
    Article Snippet: .. The following cell lines, antibodies and chemicals were used in this study: LM3, 97H, 97 L, PLC/PRF/5 (Typical Cultures Preserved Cell Bank, Chinese Academy of Sciences), anti-HPX (Abclonal, A9133, Wuhan, China), anti-GAPDH (Abclonal, A19056, Wuhan, China), antiβ-actin (Starter, S0B0734, Hangzhou, China), anti-p-Braf (CST, 2696, Danfoss, USA), anti-Braf (CST, 14814, Danfoss, USA), anti-p-MEK1/2 (Abclonal, AP1349, Wuhan, China), anti-MEK1/2 (Abclonal, A4868, Wuhan, China), anti-p-ERK1/2 (Abclonal, AP0974, Wuhan, China), anti-ERK1/2 (Abclonal, A4782, Wuhan, China), HRP-coupled goat antirabbit IgG (H+L) (Abclonal, AS014, Wuhan, China), CCK8 assay kit (Beyotime, C0038, Shanghai, China), BeyoClick EdU-488 Cell Proliferation Assay Kit (Beyotime, C0071STM, Shanghai, China), the Whole Protein Extraction Kit (KeyGEN BioTECH, KGB5303–100, Nanjing, China), diethyl phthalate (Merck, 524972, darmstadt, Germany), dimethyl phthalate (Merck, 525081, Darmstadt, Germany), Dioctyl phthalate (Merck, D201154, Darmstadt, Germany), anti-p-MEK1/2 (HUABIO, ET1609–50, Hangzhou, China), anti-p-ERK1/2 (HUABIO, ET1610–13, Hangzhou, China), anti-JNK (HUABIO, ET1601–28, Hangzhou, China), anti-p-JNK (HUABIO, ET1609–42, Hangzhou, China), anti-p38 (HUABIO, ET1702–65, Hangzhou, China), anti-p-p38 (HUABIO, HA722669, Hangzhou, China), MG132 (MCE, HY-13259, New Jersey, USA), bortezomib (MCE, HY-10227, New Jersey, USA), HCQ (MCE, HY-B1370, New Jersey, USA), Bafilomycin A1 (MCE, HY-100558, New Jersey, USA). ..

    Protein Extraction:

    Article Title: Phthalates promote lenvatinib resistance in hepatocellular carcinoma through the HPX-MAPK pathway.
    Article Snippet: .. The following cell lines, antibodies and chemicals were used in this study: LM3, 97H, 97 L, PLC/PRF/5 (Typical Cultures Preserved Cell Bank, Chinese Academy of Sciences), anti-HPX (Abclonal, A9133, Wuhan, China), anti-GAPDH (Abclonal, A19056, Wuhan, China), antiβ-actin (Starter, S0B0734, Hangzhou, China), anti-p-Braf (CST, 2696, Danfoss, USA), anti-Braf (CST, 14814, Danfoss, USA), anti-p-MEK1/2 (Abclonal, AP1349, Wuhan, China), anti-MEK1/2 (Abclonal, A4868, Wuhan, China), anti-p-ERK1/2 (Abclonal, AP0974, Wuhan, China), anti-ERK1/2 (Abclonal, A4782, Wuhan, China), HRP-coupled goat antirabbit IgG (H+L) (Abclonal, AS014, Wuhan, China), CCK8 assay kit (Beyotime, C0038, Shanghai, China), BeyoClick EdU-488 Cell Proliferation Assay Kit (Beyotime, C0071STM, Shanghai, China), the Whole Protein Extraction Kit (KeyGEN BioTECH, KGB5303–100, Nanjing, China), diethyl phthalate (Merck, 524972, darmstadt, Germany), dimethyl phthalate (Merck, 525081, Darmstadt, Germany), Dioctyl phthalate (Merck, D201154, Darmstadt, Germany), anti-p-MEK1/2 (HUABIO, ET1609–50, Hangzhou, China), anti-p-ERK1/2 (HUABIO, ET1610–13, Hangzhou, China), anti-JNK (HUABIO, ET1601–28, Hangzhou, China), anti-p-JNK (HUABIO, ET1609–42, Hangzhou, China), anti-p38 (HUABIO, ET1702–65, Hangzhou, China), anti-p-p38 (HUABIO, HA722669, Hangzhou, China), MG132 (MCE, HY-13259, New Jersey, USA), bortezomib (MCE, HY-10227, New Jersey, USA), HCQ (MCE, HY-B1370, New Jersey, USA), Bafilomycin A1 (MCE, HY-100558, New Jersey, USA). ..



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    JD-02 inhibits HSV-1 replication by suppressing the Raf/MEK/ERK signaling pathway. (A) Western blot analysis was conducted to assess the effects of HSV-1 (MOI=0.1) infection on the protein levels of BRAF, MEK and ERK, with and without treatment using JD-02. (B) Treatment with JD-02 at the specified concentration influences the protein levels of BRAF, MEK and ERK in HaCaT cells over a 12-h period. (C and D) HaCaT cells were subjected to transfection with either N.C. siRNA or ERK siRNA for a period of 48 h. Subsequently, the cells were infected with HSV-1 (MOI=0.1) for an additional 24 h. The DNA copy number of the viral gene UL54, as well as the viral protein expression of gB, ICP0, ICP27, ERK and p-ERK were evaluated. (E) The DNA copy numbers of the viral genes UL30 , UL52 and UL54 in HaCaT cells infected with HSV-1 (MOI=0.1) and subsequently treated with either JD-02 (1 μ M) or U0126 (10 μ M) for 24 h, were quantified using reverse transcription-quantitative PCR. (F) Western blot analysis of viral proteins (gB, ICP0 and ICP27), ERK and p-ERK expression in HaCaT cell infected with HSV-1 (MOI=0.1) and treated with U0126 for indicated concentration. (G) Western blot analysis of UL30 overexpression in HaCaT cells treated with U0126. Data are presented as the mean ± SD (n=3). * P<0.05, ** P<0.01, *** P<0.01 and **** P<0.0001 compared with the HSV-1 group. HSV, Herpes Simplex Virus; MOI, multiplicity of infection; N.C., negative control; siRNA, small interfering RNA; p-, phosphorylated.

    Journal: International Journal of Molecular Medicine

    Article Title: Novel Hsp90 inhibitor JD-02 inhibits HSV-1 infection via the Raf/MEK/ERK signaling pathway

    doi: 10.3892/ijmm.2026.5810

    Figure Lengend Snippet: JD-02 inhibits HSV-1 replication by suppressing the Raf/MEK/ERK signaling pathway. (A) Western blot analysis was conducted to assess the effects of HSV-1 (MOI=0.1) infection on the protein levels of BRAF, MEK and ERK, with and without treatment using JD-02. (B) Treatment with JD-02 at the specified concentration influences the protein levels of BRAF, MEK and ERK in HaCaT cells over a 12-h period. (C and D) HaCaT cells were subjected to transfection with either N.C. siRNA or ERK siRNA for a period of 48 h. Subsequently, the cells were infected with HSV-1 (MOI=0.1) for an additional 24 h. The DNA copy number of the viral gene UL54, as well as the viral protein expression of gB, ICP0, ICP27, ERK and p-ERK were evaluated. (E) The DNA copy numbers of the viral genes UL30 , UL52 and UL54 in HaCaT cells infected with HSV-1 (MOI=0.1) and subsequently treated with either JD-02 (1 μ M) or U0126 (10 μ M) for 24 h, were quantified using reverse transcription-quantitative PCR. (F) Western blot analysis of viral proteins (gB, ICP0 and ICP27), ERK and p-ERK expression in HaCaT cell infected with HSV-1 (MOI=0.1) and treated with U0126 for indicated concentration. (G) Western blot analysis of UL30 overexpression in HaCaT cells treated with U0126. Data are presented as the mean ± SD (n=3). * P<0.05, ** P<0.01, *** P<0.01 and **** P<0.0001 compared with the HSV-1 group. HSV, Herpes Simplex Virus; MOI, multiplicity of infection; N.C., negative control; siRNA, small interfering RNA; p-, phosphorylated.

    Article Snippet: These primary antibodies included ICP0 (1:1,000; cat. no. ab6513; Abcam), ICP27 (1:1,000; cat. no. ab53480; Abcam), gD (1:1,000; cat. no. ab6507; Abcam), gB (1:500; cat. no. sc-56987; Santa Cruz Biotechnology, Inc.), Raf-B (1:500; cat. no. sc-166; Santa Cruz Biotechnology, Inc.), p-BRAF (1:1,000; cat. no. 2696T; Cell Signaling Technology, Inc.), ERK (1:1,000; cat. no. 4695S; Cell Signaling Technology, Inc.), p-ERK (1:1,000; cat. no. 4370S; Cell Signaling Technology, Inc.), MEK1/2 (1:1,000; cat. no. AF6385; Affinity Biosciences), p-MEK1/2 (1:1,000; cat. no. 9154T; Cell Signaling Technology, Inc.), Flag (1:1,000; cat. no. 14793S; Cell Signaling Technology, Inc.), HA (1:1,000; cat. no. 3724S; Cell Signaling Technology, Inc.).

    Techniques: Western Blot, Infection, Concentration Assay, Transfection, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Over Expression, Virus, Negative Control, Small Interfering RNA

    Antibodies used in the experiment.

    Journal: Cell Death & Disease

    Article Title: OTUD4 promotes the progression of glioblastoma by deubiquitinating CDK1 and activating MAPK signaling pathway

    doi: 10.1038/s41419-024-06569-x

    Figure Lengend Snippet: Antibodies used in the experiment.

    Article Snippet: p-BRAF(T401) , bs-5224R , , , BIOSS , Beijing, China.

    Techniques: Western Blot